Author/Authors :
Yaghoobi, Hajar Clinical Biochemistry Research Center - Basic Health Sciences Institute - Shahrekord University of Medical Sciences, Iran , Azizi, Hakim Department of Medical Parasitology - School of Medicine - ZabolUniversity of Medical Sciences, Iran , Banitalebi-Dehkordi, Mehdi Clinical Biochemistry Research Center - Basic Health Sciences Institute - Shahrekord University of Medical Sciences, Iran , Mohammad Rezaei, Fatemeh Department of Medical Genetic - Faculty of Medicine - Tabriz University of Medical Sciences, Iran , Arsang-Jnag, Shahram Clinical Research Development Center (CRDU) - Qom University of Medical Sciences, Iran , Taheri, Mohammad Urogenital Stem Cell Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ghafouri-Fard, Soudeh Department of Medical Genetics - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Background: The enzyme beta-secretase 1 (BACE1) and its antisense transcript (BACE1-AS) have been
implicated in the pathogenesis of Alzheimer's disease. Moreover, several lines of evidence point to their
contribution in tumorigenesis.
Methods: In the present study, we evaluated expression of BACE1 mRNA (BACE1) and BACE1-AS in 54
breast cancer tissues and 54 adjacent non-cancerous tissues (ANCTs) from the same patients using quantitative
real-time PCR.
Results:BACE1 was significantly down-regulated in tumoral tissues compared with ANCTs, while BACE1-
AS expression was not significantly different between tumoral tissues and ANCTs. The Bayesian Multilevel
model showed a significant difference in BACE1 expression between stage 1 and 2 cancers after age-effect
adjustments. BACE1-AS expression was significantly greater in ER-positive than in ER-negative samples
(P=0.01). BACE1 and BACE1-AS expression were not correlated with patient ages in any sample sets.
Conclusions:Significant correlations were detected between expression of these genes in both tumoral tissues
and ANCTs. The current study provides evidence for differential BACE1 expression in breast tissues and
suggests further assessment of the role of BACE1 in the pathogenesis of cancer.
Keywords :
BACE1 , BACE1-AS , Breast Cancer , Lncrna
