Title of article :
Introducing the Best Six Loci in Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat (MIRU-VNTR) Typing for Mycobacterium Tuberculosis Genotyping
Author/Authors :
Ghavidel, Mahdis Antimicrobial Resistance Research Center - Mashhad University of Medical Sciences, Iran , Tadayon, Keyvan Department of Microbiology - Razi Vaccine and Serum Research Institute (RVSRI) - Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran , Mosavari, Nader Department PPD Tuberculin - Razi Vaccine and Serum Research Institute, (RVSRI) - Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran , Nourian, Kimiya School of Veterinary Medicine - Ferdowsi University of Mashhad, Iran , BahramiTaghanaki, Hamid Reza School of Persian Medicine - Mashhad University of Medical Sciences, Iran , Mohammadi, Gholam Reza Department of Clinical Sciences - School of Veterinary Medicine - Ferdowsi University of Mashhad, Iran , Rashtibaf, Mohammad Veterinary administration of Khorasan Razavi Province, Mashhad, Iran , Ghazvini, Kiarash Antimicrobial Resistance Research Center - Mashhad University of Medical Sciences, Iran
Abstract :
Background:Tuberculosis (TB) still remains endemic worldwide making epidemiological studies essential to
mitigating efforts implicated in identifying its source, controlling, and preventing the spread of dangerous
strains amongst humans such as Mycobacterium tuberculosis (Mtb).
Methods: In this study, we sought to determine the 6 Mycobacterial Interspersed Repetitive Unit-VariableNumber Tandem Repeat (MIRU-VNTR) loci with high discriminatory powers for Mtb genotyping as well
as the loci with the highest and the lowest discriminatory powers for MIRU-VNTR. To conduct our search,
we used several databases such as science direct, Embase (Elsevier), Web of Science, Scopus and Medline
via PubMed. Searches were performed using key words including: Mycobacterium tuberculosis, MIRU–
VNTR, Allele diversity, Genetic diversity and human patient. Finally, 56 articles were selected after filtering
out titles, abstracts and full texts.
Results: Loci with high discriminatory powers included MIRU10 and MIRU26, while MIRU2, MIRU20,
MIRU24 and ETRD had poor discriminatory powers. According to previous data in the literature, the loci
MIRU10, MIRU26, MIRU40, QUB 26, QUB 11b and Mtub21 have high discriminatory powers.
Conclusions: Therefore, these loci recommended for genotyping Mtb to save time and cost and to ensure the
production of reliable results.
Keywords :
Discriminatory power , Genotyping , MIRU-VNTR , Mycobacterium tuberculosis
Journal title :
Reports of Biochemistry and Molecular Biology (RBMB)
