Title of article :
The Protective Role of Gallic Acid Pretreatment On Renal Ischemia-reperfusion Injury in Rats
Author/Authors :
Ahmadvand, Hassan Razi Herbal Medicine Research Center - Faculty of Medicine - Lorestan University of Medical Sciences, Khorramabad, Iran , Yalameha, Banafsheh Student Research Committee - Lorestan University of Medical Sciences, Khorramabad, Iran , Adibhesami, Glavizh Departmentof Biochemistry - Faculty of Medicine - Lorestan University of Medical Sciences, Khorramabad, Iran , Nasri, Maryam Student Research Committee - Lorestan University of Medical Sciences, Khorramabad, Iran , Naderi, Negar Student Research Committee - Lorestan University of Medical Sciences, Khorramabad, Iran , Babaeenezhad, Esmaeel Department of Clinical Biochemistry - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Nouryazdan, Negar Departmentof Biochemistry - Faculty of Medicine - Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract :
Background:Renal ischemia-reperfusion injury (RIR) occurs when there is a temporary restriction of blood flow to
the kidneys followed by an influx of blood, re-oxygenating the tissues. This occurs as a severe complication of major
surgery. This process causes significant damage to the tissues and is responsible for the development of acute kidney
injury (AKI), a life-threatening condition with high mortality rates. Here, we evaluated the potential protective effects
of the antioxidant, gallic acid (GA), on RIR in an in vivo rat model.
Methods:Adult male Sprague Dawley rats were randomly divided into three groups: group 1 (control, n = 8), group 2
(Ischemia-reperfusion (IR) with no-treatment, n = 7), and group 3 (IR + daily GA 100 mg/kg i.p, n = 7). The abdomens
of the rats in the control group were opened during the surgical procedure, then sutured closed. GA pretreatment began
daily 15 days prior to inducing RIR. To induce RIR, the umbilical arteries were obstructed on both sides and clamped
with mild pressure for 45 min. Following the 45 min ischemia, the clamps were removed to allow for the induction of
reperfusion. The reperfusion phase was 24 hours.
Results: Following IR, the serum levels of urea and creatinine significantly increased compared to the
controls. Pretreatment with GA was observed to reduce urea and creatinine levels following IR. However,
this decrease was not statistically significant. The serum and renal levels of malondialdehyde (MDA) in the
IR group was significantly elevated compared to the control group. Conversely, glutathione (GSH) levels
and the activity of glutathione peroxidase (GPX) significantly decreased in the IR group compared to
controls. Our findings show GA pretreatment to significantly improve the levels of renal MDA, serum GSH,
and GPX activity following RIR.
Conclusions: Our findings highlight the protective role for GA in mitigating the damage caused by RIR and its
applications as a potential treatment.
Keywords :
Antioxidant enzymes , Gallic acid , Renal functional markers , Renal ischemia-reperfusion
Journal title :
Reports of Biochemistry and Molecular Biology (RBMB)
