Author/Authors :
Chashmniam, Saeed Department of Chemistry - Sharif University of Technology , Ghafourpour, Mohsen 22 Bahman Hospital - Neyshabur University of Medical Sciences , Rezaei Farimani, Azam Noncommunicable Diseases Research Center - Neyshabur University of Medical Sciences , Gholami, Ali Noncommunicable Diseases Research Center - Neyshabur University of Medical Sciences , Nobakht Motlagh Ghoochani, Bibi Fatemeh Noncommunicable Diseases Research Center - Neyshabur University of Medical Sciences
Abstract :
Background: Nonalcoholic fatty liver disease (NAFLD) is the most abundant chronic liver disorder, because racial and ethnic differences may influence prevalence and severity of NAFLD.
Objectives: This metabolomic study was conducted to identify the metabolic biomarkers and determine the mechanism of
progress of NAFLD in Iranian patients.
Methods: Serum samples were collected from 75 participants (37 healthy controls and 38 patients with NAFLD) after an overnight
fast. The metabolome of all samples were determined by nuclear magnetic resonance (NMR) and were compared by multivariate
statistical analysis.
Results: Totally, 19 metabolomic biomarkers were identified by NMR. Compared to healthy controls, NAFLD patients had increased
serum concentrations of glycochenodeoxycholic acid, taurocholic acid, glycocholic acid, deoxycholic acid, valine, isoleucine, succinic acid, isocitric acid, 2-ketoglutaric acid, trimethylamine, proline, hydroxyproline and tyrosine, while the concentrations of
butyric acid, propionic acid, isovaleric acid, glutamine, glycine, and serine decreased.
Conclusions: A robust set of biomarkers for diagnosis of NAFLD was established. Serum metabolomics biomarkers revealed
changes in some amino acids and their derivatives, bile acids, short chain fatty acids, and tricarboxylic acid cycle intermediates in
subjects with NAFLD compared to healthy controls. These markers could be used as indicators regarding the efficacy of therapeutic
interventions.
