Food Safety Evaluation of Imidacloprid Residues in Grape Berries at a Different Dose of Spraying

Background Aims of the Study: Grape, a crucial agriculture crop of Malayer, is affected by Vine cicada, Psalmocharias alhageos. Imidacloprid, a neonicotinoid insecticide, provides good management of this insect. The aim of the current study was to residue persistence of imidacloprid on grapes, to estimate its residue deposit, the half-life of degradation and safe pre-harvest consumption time. Materials Methods: Residues of imidacloprid were estimated in grape following two spraying of recommended (80.0 g a.i. ha^-1) and doubles the application rate (160.0 g a.i. ha^-1). Samples were collected at 1 h to 21 days after spraying of imidacloprid. The analyses were done by the (QuECHERS) technique using HPLC-UV. Results: The average initial concentrations of imidacloprid on grapes were found to be 10.58 and 17.56 mg kg-1 at single and double dosages, respectively. These residues of imidacloprid decreased to be the extract of 97.8% and 98.0%, respectively, at single and double dosages in 15 days, with a half-life period of 2.21 and 2.94 days. Residues of imidacloprid on grapes were less than its MRL value after 7 and 10 days of it’s spraying at the recommended and double dosage. Residues of imidacloprid in grape berries at harvest were discovered to below the determination limit. Conclusions: Consequently, a waiting time of 7 and 10 days is usually recommended for safe consumption of grapes once imidacloprid spraying. Acceptable daily intake (ADI) of imidacloprid is 0.06 mg kg^-1 body weight day^-1. According to the results of this study, the employment of imidacloprid on the grape crop looks to be toxicologically acceptable.