Author/Authors :
Karami, Ali Baqiyatallah University of Medical Sciences, Tehran , Tebyanian, Hamid Baqiyatallah University of Medical Sciences, Tehran , Sayyad Soufdoost, Reza Imam Khomeini Clinic of Dentistry, Tehran , Motavallian, Ebrahim Department of General Surgery - Faculty of Medicine - Baqiyatallah University of Medical Sciences, Tehran , Barkhordari, Aref Baqiyatallah University of Medical Sciences, Tehran , Nourani, Mohammad Reza Baqiyatallah University of Medical Sciences, Tehran
Abstract :
BACKGROUND
Collagen is the main product in pharmaceutics and food industry
with a high demand. Collagen can be extracted from several
tissues such as skin, bone and tendon, etc. Collagen can be
used in tissue engineering researches as a substrate of wound
healing and nerve regeneration. Extraction methods of collagen
are various with different purities. In this research, we aimed to
extract collagen from human placenta with a modified method.
METHODS
This modified approach was used for extracting of collagen
from human placenta with acetic acid and NaCl treatment using
different concentrations.
RESULTS
SDS page showed three different bands that reflected two alphachains
and one beta-chain with molecular weights of 102, 118
and 220 kDa, respectively. There was no significant difference
between extracted collagen from human placenta and standard
collagen in western blot analysis.
CONCLUSION
It was concluded that human placenta can be an alternative source
of collagen with high purity for biomedical applications such as
tissue engineering, stem cell therapy and research.
Keywords :
Collagen , Huma , Placenta , Extraction , Characterization
