Author/Authors :
Ayoub, Shymaa E. Department of Biochemistry and Molecular Biology - Fayoum University, Al Fayoum, Egypt , Shaker, Olfat G. Department of Medical Biochemistry and Molecular Biology - Cairo University, Giza, Egypt , Abdelwahed, Mostafa Y. Department of Physiology - Fayoum University, Al Fayoum, Egypt , Ahmed, Naglaa A. Department of Physiology - Zagazig University, El Zagazig, Egypt , Abdelhameed, Hazem G. Department of Gynecology and Obstetrics - Fayoum University, Al Fayoum, Egypt , Bosilah, Almandouh H. Department of Gynecology and Obstetrics - Fayoum University, Al Fayoum, Egypt , Mohammed, Sheren R. Department of Biochemistry and Molecular Biology - Fayoum University, Al Fayoum, Egypt
Abstract :
Preeclampsia (PE) is a multifactorial disorder. Several studies showed that micro RNAs may play a critical role in PE pathogenesis. We aimed to investigate for the first time the association of mir-155rs767649 polymorphism with PE. Eighty patients with preeclampsia and 80 normal subjects were enrolled in the study. Serum expression levels of mature mir-155were evaluated using real-time PCR, and mir-155 rs767649 (T/A) polymorphism was genotyped using TaqMan SNP genotyping. There was a significant difference between the expression level of mir-155 in cases (5.86 ± 3.11) in comparison with controls (0.58 ± 0.30) (P<0.0001). Also,the minor allele of rs767649 was significantly associated with increased risk of PE [Recessive model: adjusted Odds ratio (OR) = 5.240, 95% confidence interval (CI) = (1.999-13.733),P= 0.001]. There was a significant difference between different genotypes according to expression levels of mir-155 in PE (P<0.0001) with high expression levels in TA genotype (7.10 ± 3.11 ). Mir-155 may play a critical role in PE pathogenesis. The obtained data suggest that a minor allele of rs767649 might be a predisposing factor for PE.
Keywords :
MicroRNA , mir-155 , single nucleotidepolymorphism , preeclampsia
