Author/Authors :
Widowati, Wahyu Medical Research Center - Maranatha Christian University, Bandung, West Java, Indonesia , Noverina, Rachmawati PT Bio Farma (Persero), Bandung, West Java, Indonesia , Ayuningtyas, Wireni PT Bio Farma (Persero), Bandung, West Java, Indonesia , Kurniawan, Dedy PT Bio Farma (Persero), Bandung, West Java, Indonesia , Widya Kusuma, Hanna Sari Biomolecular and Biomedical Research Center - Aretha Medika Utama, Bandung, West Java, Indonesia , Arumwardana, Seila Biomolecular and Biomedical Research Center - Aretha Medika Utama, Bandung, West Java, Indonesia , Artie, Dwi Surya Biomolecular and Biomedical Research Center - Aretha Medika Utama, Bandung, West Java, Indonesia , Sholihah, Ika Adhani Biomolecular and Biomedical Research Center - Aretha Medika Utama, Bandung, West Java, Indonesia , Handayani, Anisa Siwianti Biomolecular and Biomedical Research Center - Aretha Medika Utama, Bandung, West Java, Indonesia , Laksmitawati, Dian Ratih Pancasila University, Jakarta, Indonesia , Rinendyaputri, Ratih National Institute of Health Research and Development - Ministry of Health, Jakarta, Indonesia , Rilianawati, Rilianawati Agency for the Assesment and Application of Technology - Ministry of Research and Technology, Serpong, Indonesia , Faried, Ahmad Department of Neurosurgery and Stem Cell Working Group - Universitas Padjadjaran, Bandung, West Java, Indonesia
Abstract :
Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research aimed to determine the proliferation, characteristics and differentiation capacity of adipose tissue-derived (AT)-MSCs at many passages with different media. The cell proliferation capacity was assayed using trypan blue. MSCs characterization (CD90, CD44, CD105, CD73, CD11b, CD19, CD34, CD45, and HLA-DR) was performed by flow cytometry, and cell differentiation was determined by specific stainings. Population doubling time (PDT) of AT-MSCs treated with fresh frozen plasma (FFP) and non-FFP increased in the late passage (P) (P15 FFP was 22.67 ± 7.01 days and non-FFP was 19.65 ± 2.27 days). Cumulative cell number was significantly different between FFP and non-FFP at P5, 10, 15. AT-MSCs at P4-15 were positive for CD90, CD44, CD105, and CD73, and negative for CD11b, CD19, CD34, CD45, and HLA-DR surface markers. AT-MSCs at P5, 10, 15 had potential toward adipogenic, chondrogenic, and osteogenic differentiation. Therefore, PDT was affected by increased age but no difference was observed in morphology, surface markers and differentiation capacity among passages. Cumulative cell number in FFP was higher in comparison with non-FFP in P5, 10, 15. Our data suggest that FFP may replace FBS for culturing MSCs.
