Author/Authors :
Moghimi, Mansour Department of Pathology - Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Rashidian, Sara Department of Medical Immunology Sciences - Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Khosravian, Farinaz Molecular, Cellular and Genetics Research Center, Isfahan, Iran , Hadi, Nasrin Medical Genetics Research Center of Genome, Isfahan, Iran
Abstract :
Background: Liver cancer is the second most common reason for cancer-related death
globally, and thus, a major public health challenge. Plant-based drugs are promising
therapeutic agents against cancers. Basil and Dracocephalum kotschyi extracts also
exhibit therapeutic impact on various cancers. To assess the effects of the extract of
Basil and Dracocephalum kotschyi on cell proliferation and BCL2 expression in the
HepG2 cell line.
Materials and Methods: In this experimental study, the HepG2 cell line was selected
for treatment with the alcoholic extracts of Basil and Dracocephalum kotschyi. Cell
proliferation was examined in the presence of various concentrations of the Basil and
Dracocephalum kotschyi extracts by MTT assay. Moreover, the effect of the alcoholic
extracts of Basil and Dracocephalum kotschyi on BCL2 expression was evaluated
using quantitative real-time polymerase chain reaction (qRT-PCR).
Results: Cell proliferation analyses showed anticancer characteristics of the alcoholic
extracts of Basil and Dracocephalum kotschyi. In addition, treatment with the extracts
of Basil and Dracocephalum kotschyi led to the decreased expression of BCL2 in
HepG2 cell line.
Conclusion: These findings indicated that Basil and Dracocephalum kotschyi are
promising candidates for future anticancer research.
Keywords :
Basil , BCL2 , Dracocephalum kotschyi , HepG2 , Liver cancer
