Author/Authors :
Taghadosi ,Camellia Department of Clinical Sciences - Shahrekord University, Shahrekord, Iran , Kojouri, Gholam Ali Department of Clinical Sciences - Shahrekord University, Shahrekord, Iran , Ahadi, Ali Mohammad Department of Genetics - Shahrekord University, Shahrekord, Iran , Hashemi Bahremani, Mohammad Laboratory of Pathology - Imam Hossein Hospital, Tehran, Iran , Kojouri, Abdolnabi International Campus of Shahid Sadoughi Medical University, Yazd, Iran
Abstract :
Background: Bovine Leukaemia Virus (BLV) is the cause of Enzootic Bovine Leukosis (EBL) which belongs to retroviruses including Human T-cell leukaemia virus and simian T-lymphotropic virus. Due to this familiarity, the possibility of BLV transfer from animal production to humans may exist.
Materials and Methods: In the present study, formalin-fixed, paraffin-embedded and fresh human lymphomas tissues were used for detecting the BLV Tax genome and BLV Tax expression using nested-PCR and RT-nested-PCR.
Results: Nested-PCR evaluation showed that 9 of 41 samples contained Tax region of BLV genome, of which eight samples belonged to high grade diffuse large cell lymphoma (B-cell type). Also, the results of RT-nested-PCR showed the BLV Tax expression in 2 of 5 high grade diffuse large cell lymphoma (B-cell type) samples.
Conclusion: These findings explain the possible relation between BLV infection and the occurrence of some types of human lymphomas for the first time.
Keywords :
Lymphoma , Nested PCR , RTnested- PCR , Tax
