Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1 Tumor Cell Lineage

Background: Cancer is amongst the leading causes of death all over the world. Breast cancer is responsible for the largest number of deaths among women. Several studies confirm that Bifidobacterium bifidum as a probiotic significantly inhibits breast cancer development. The present study aimed to investigate the effect of B. bifidum supernatant on the cell growth inhibition of the breast cancer 4T1 cell line in vitro. Materials and Methods: The present experimental work was conducted at Mazandaran University of Medical Sciences, Sari City, Iran. B. bifidum was cultured in the de Man Rogosa, and Sharpe broth at 37° C for 72 h anaerobically and the B. bifidum Supernatant (BS) was prepared by the freezingthawing procedure. The cell growth inhibition of the probiotic strain was assessed using the MTT assay through breast cancer (4T1) cell line. Results: The results showed that the supernatant extracted from B. bifidum strain had good antiproliferative effects against 4T1 cancer cell line, compared with the control group. The inhibitory effects are enhanced by passing the time. Conclusion: B. bifidum supernatant could be a potential probiotic candidate for the treatment of breast cancer. However, further in vitro and in vivo studies are required to support our initial findings.