Title of article :
Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation
Author/Authors :
Gu ، Jianhong Joint International Research Laboratory of Agriculture and Agri-Product Safety - College of Veterinary Medicine - Ministry of Education of China,Yangzhou University , Tong ، Xishuai College of Veterinary Medicine - Yangzhou University , Chen ، Yang College of Veterinary Medicine - Yangzhou University , Zhang ، Chuang College of Veterinary Medicine - Yangzhou University , Ma ، Tianhong College of Veterinary Medicine - Yangzhou University , Li ، Saihui College of Veterinary Medicine - Yangzhou University , Min ، Wenyan College of Veterinary Medicine - Yangzhou University , Yuan ، Yan College of Veterinary Medicine - Yangzhou University , Liu ، Xuezhong College of Veterinary Medicine - Yangzhou University , Bian ، Jianchun College of Veterinary Medicine - Yangzhou University , Liu ، Zongping Joint International Research Laboratory of Agriculture and Agri-Product Safety - College of Veterinary Medicine - Ministry of Education of China, Yangzhou University
Abstract :
Background: Vitamin D is an important steroid that can regulate bone metabolism including osteoclast (OC) differentiation. Transient receptor potential cation channel subfamily V member 5 (TRPV5), is a calcium channel protein involved in OC differentiation. However, the impact of vitamin D on TRPV5 expression during OC differentiation is not clear. Objectives: To determine if 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates the expression of TRPV5 during OC differentiation. Methods: Bone marrow mononuclear macrophage (BMMs) were induced to differentiate intoOCwith or without treatment with 10 nM1,25(OH)2D3. The expression levels of vitamin D receptor (VDR) and TRPV5 were examined. The expression of several OC markers, including tartrate resistant acid phosphatase (TRAP), carbonic anhydrase II (Ca II), cathepsin K (CTSK), and vacuolar-type H+-ATPase (V-ATPase) were also detected. Results:We found that theVDRwas expressed in murine bonemarrow-derived macrophages at the early stage of OCdifferentiation. TRPV5 expression was increased during OC differentiation, which was down-regulated by 1,25(OH)2D3 after a prolonged exposure. The 1,25(OH)2D3 and TRPV5 inhibitors inhibited OC differentiation. Conclusions: 1,25(OH)2D3 can inhibit TRPV5 expression as well as TRPV5 inhibitors during OC differentiation. This suggests that 1,25(OH)2D3 may suppress OC differentiation by inhibiting TRPV5 expression.
Keywords :
Osteoclast , 1 , 25(OH)2D3 , TRPV5 , RANKL , Ca^2+
Journal title :
International Journal of Endocrinology and Metabolism
Journal title :
International Journal of Endocrinology and Metabolism
