Author/Authors :
Nematollahi, Parisa Shiraz University of Medical Sciences - Student Research Committee , Mohammadi Aref, Nasrin Islamic Azad University, Tehran Medical Sciences Branch - Student Research Committee , Zahmatkesh Meimandi, Farzaneh Shiraz University of Medical Sciences - Student Research Committee , Rozei, Laya Shiraz University of Medical Sciences - Student Research Committee , Zareé, Hamidreza Shiraz University of Medical Sciences - Student Research Committee , Mirlohi, Mohammad Javad Islamic Azad University, Tehran Medical Sciences Branch - Student Research Committee , Rafiee, Shima Shiraz University of Medical Sciences - Student Research Committee , Mohsenikia, Maryam Islamic Azad University, Tehran Medical Branch - Young Researchers and Elite Club , Soleymani, Ali Islamic Azad University, Tehran Medical Branch - Young Researchers and Elite Club , Ashkani-Esfahani, Soheil Shiraz University of Medical Sciences - Student Research Committee , Ebrahimi, Alireza Shiraz University of Medical Sciences - Student Research Committee , Noorafshan, Ali Shiraz University of Medical Sciences - Histomorphometry and Stereology Research Center
Abstract :
Background: Diabetes mellitus (DM) has a broad spectrum of complications and comorbidities. Delayed wound healing is a major
reason for infections and further life-threatening complications; the process of vascularization and tissue regeneration as well as
anti-inflammatory and anti-oxidant reactions of the body are impaired in DM. Matricaria Chamomilla (MC) is a herbal medicine
which has antimicrobial, antioxidant and wound healing potentials.
Objectives: In this study, these potentials are evaluated on full thickness diabetic excisional skin wounds by using stereological
methods.
Methods: In this experimental study, 48 male Wistar rats with streptozotocin (STZ)-induced diabetes (50 mg/kg intraperitoneally)
were divided into 4 groups (n = 12) randomly: MC 5% gel treated, MC 10% gel treated, gel-base treated group, and the control group
receiving no treatment. After creating 1 cm2 wounds, rats were treated every 24 hours for 15 days. Volume densities of collagens,
wound closure rate, volume density, length density, and mean diameter of vessels, and fibroblast proliferation, were examined and
analyzed. P0.05 was mentioned as statistically significant.
Results: MC gels receiving groups showed hastened wound area reduction in contrast with gel-base and control groups (P < 0.05).
Numerical density of fibroblasts, mean diameter and length densities of the vessels inMCgroups were significantly higher than the
control and the base groups (P < 0.05).
Conclusions: It is demonstrated that MC has the ability to improve wound healing by enhancing fibroblast proliferation and revascularization
in diabetic skin injuries and has the potential to be considered as an alternative treatment.
Keywords :
Diabetes Mellitus , Wound , Matricaria Chamomilla , Stereology , Rat
