Frequency of Y Chromosome Microdeletions in Azoospermic and Oligospermic Iranian Infertile Men

Background and Aims:Azoospermia factor (AZF)region of the Y-chromosome has several genes which areresponsible for normal spermatogenesis. Microdeletionsof these genes are associated with azoospermia and oligospermia. These microdeletions are too small to be detected by karyotyping. They canbe easily identified using polymerase chain reaction.The aim of this study is to determine the frequencies of Y-chromosome microdeletions inazoospermic and oligospermic Iranian infertile men and comparethemwith other studiesin different ethnic groups.Materials and Methods: At first, karyotype analysiswas performed in 80 infertile men and 30 healthy age-matched counterpartsas control groupusing standard cytogenetic methods.Second, genomic DNA was extracted from all casesand genetic screeningwas conductedfor Y chromosome microdeletions by multiplex polymerase chain reaction for AZF genes on both infertile and control men using 6 STS markers on the long arm of the Y chromosome.Results:Totally,49 infertile men were azoospermic and 31 were oligospermic. Y-chromosome microdeletions in the AZFc region were detected in 4 of azoospermic patients. Y-chromosome microdeletions was not detected in any of the oligospermic patients and the control group.Conclusions:This findingrecommends that genetic counseling and screening before starting assisted reproductive techniques such as in vitro fertilisation and intracytoplasmic sperm injectioncan prevent unnecessary treatment and transmission of genetic defects to offspring.