Title of article :
A Quick and Simple Polarographic Method for Aluminum Measurement in Recombinant Hepatitis B Vaccine
Author/Authors :
Alebouyeh ، Mahmoud Department of Food and Drug - Laboratory Research Center - Ministry of Health and Medical Education , Abedi ، Zahra Department of Analytical Chemistry, Biology Unit of Food and Drug Administration for Quality Control - Food and Drug Administration - Ministry of Health and Medical Education , Rastegar ، Hossein Department of Pharmacology - Food and Drug Administration, Health and Cosmetics Research Center - Ministry of Health and Medical Education , Bagheri ، Hasan Department of Analytical Chemistry - Faculty of Engineering - Islamic Azad University, Research and Sciences Branch , Vaez ، Javad Department of Chemistry - Pasteur Institute of Iran , Akbari-Adergani ، Behrouz Department of Analytical Chemistry - Food and Drug Administration, Food and Drug Laboratory Research Center - Ministry of Health and Medical Education
Abstract :
Background and Objective: Aluminum salts are among the most common useful additive compounds in preparation of human and animal vaccines. Aluminum phosphate and aluminum hydroxide are two additives that show good immunoadjuvant effects with many antigens. Aluminumcontaining vaccines lead to a better and longer immune response compared to adjuvantlacking vaccines. The Chromogenic methods used for determination of aluminum amounts in manufacturing centers are timeconsuming and requires some experienced technicians to obtain accurate results. This study aimed to design and validate a simple polarographic method to measure aluminum in recombinant hepatitis B vaccine. Methods: In this study, the effects of temperature, pH, potential range and potential scan rate on the polarographic method of measuring aluminum in hepatitis B vaccine was evaluated and the optimal values for each of these factors were achieved. Results: In order to measure aluminum, temperature of 60 deg;C and pH of 4.5 were found as the optimal values. Implementation of polarographic method in the potential range of 0.25 to 0.1 volts had a better signal. Conclusion: Since the polarography method is more simple, accurate and faster than the chromogenic methods, it is suitable to be used for the measurement of aluminum in hepatitis B vaccine and it is recommended to be used in quality control laboratories for biological products.
Keywords :
Adjuvant , Hepatitis B Vaccine , Polarography , Aluminum.
Journal title :
Medical Laboratory Journal
Journal title :
Medical Laboratory Journal
