Title of article :
Inhibitory Potential of Metabolites from Lactobacillus casei against Phenotypic Characteristics of Salmonella typhimurium LT2
Author/Authors :
Naghili ، Hossein Dr Pourmehdi Food and Quality Control Laboratory , Tajik ، Hossein Department of Food Hygiene and Quality Control - Faculty of Veterinary Medicine - Urmia University , Raeisi ، Mojtaba Department of Health - Faculty of Health - Golestan University of Medical Sciences , Ghasem Mahdi ، Hadi Department of Food Hygiene and Quality Control - Faculty of Veterinary Medicine - Urmia University , Moradi ، Mehran Department of Food Hygiene and Quality Control - Faculty of Veterinary Medicine - Urmia University , Amin zare ، Majid Department of Food Hygiene and Quality Control - Faculty of Veterinary Medicine - Urmia University , Mehdizadeh ، Touraj Department of Food Hygiene and Quality Control - Faculty of Veterinary Medicine - Urmia University , Hasanzadazar ، Hasan Department of Food Hygiene and Quality Control - Zanjan University of Medical Sciences , Hariri ، Fardin Nezaja Department of Health
Abstract :
Background and Objective: Several studies have shown the antimicrobial activity of lactobacilli against Salmonella enterica (serotype typhimurium). The aim of this study was to evaluate the inhibitory potential of metabolites produced by probiotic culture of Lactobacillus casei against S. typhimurium and its impact on S. typhimurium motility and biofilm formation. Methods: In order to evaluate the impact of the metabolites, L. casei cell-free culture supernatant (CFCS) was collected by centrifugation of L. casei secondary cultures. Effectiveness of the CFCS against Salmonella was evaluated by the well-diffusion method. In addition, in vitro effect of this treatment on motility in Swarm agar and biofilm formation by the bacteria was investigated. Results: Inhibition zone diameters of S. typhimurium were 0.83 and 12.1 mm at concentrations of 50 and 100 μl of Lactobacillus CFCS against the log4 of S. typhimurium, respectively. Moreover, CFCS treatment inhibited the motility and biofilm formation by Salmonella. Concentrations of 5% and 10% were determined as the minimum inhibitory concentrations for motility and biofilm formation by S. typhimurium. Furthermore, effectiveness of the CFCS against Salmonella was dose-dependent (P 0.05). Conclusion: L. casei CFCS is able to inhibit the growth, motility and biofilm formation in S. typhimurium.
Keywords :
Anti , Bacterial , Lactobacillus Casei Metabolites , Phenotypic Characteristics of S. typhimurium
Journal title :
Medical Laboratory Journal
Journal title :
Medical Laboratory Journal
