Role of Pea Protein Hydrolysates as Antinephrotoxicity

Introduction: Renal damage can be caused by various causes. One of them is drugs that are toxic to  the  renal,  such  as  cisplatin  (CP).  In  an  attempt  to  find  a  remedy  for  antinephrotoxicity,  several  hydrolyzed proteins were investigated. This study was conducted to find out the effects of 8 peas protein  hydrolysates  (PPH)  hydrolyzed  using  simple  procedure  to  renal  organ  indexes  (OIs)  and  histopathological features of CP‑induced nephrotoxicity Wistar rats. Materials and Methods: Protein hydrolysates of yellow peas, gude beans, green peas, and pea protein isolate (PPI) which hydrolyzed using  neutrase  or  bromelain  were  administered  to  50  female Wistar  rats.The  treatments  were  given  for 30 days, and on day 7, all groups of rats, except negative control group, were injected CP intraperitoneal.  Renal  OIs  were  measured  and  kidneys  were  histopathological  analyzed,  which  the  results were converted to scoring system. Data were analyzed using ANOVA, LSD, Kruskal–Wallis, and  Mann–Whitney  test.  Results: Data of renal OIs were homogenous and normally distributed but  were  not  significantly  different  between  groups  (P  >  0.05).The  nephrotoxicity  of  CP  were  not  changing the renal OI but worsen the histopathological features of renal tubules in CP-induced rats (P  <  0.01).  All  protein  hydrolysate  treatment  groups  showed  less  histopathological  score  than CP group. Green PPH hydrolyzed by bromelain-treatment group showed the lowest scores. Conclusion: All PPH hydrolized with neutrase or bromelain improve the CP-induced nephrotoxicity rats. Green PPH with bromelain hydrolyzed had a promising potency as antinephrotoxicity.