Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer

Background: By binding SHBG hormone to sex hormones, in addition to carrying them in the blood, they regulate the amount of tissue availability. The genetic changes in the structure of globulin affect its binding to hormones, so in this study the effects of single nucleotide change in exon 8 or rs 6259 in the incidence of prostate cancer was evaluated. Materials and Methods: The study population included 120 patients with prostate cancer and 120 control subjects. After collecting blood samples, DNA was extracted by salting out procedure in order to determine the genotype of individuals by RFLP-PCR method. According to Hardy-Weinberg equilibrium genotypes the allele frequencies were calculated and a relationship between this variation and prostate cancer were evaluated using SPSS V.23.Also, the relationship between variations was investigated. P-values smaller than or equal to 0.05 were considered significant. Results: Results indicated that homozygous mutant genotype AA 2.58 (p value= 0.007, OR: 2.58, CI95%: 1.52-4.38) and heterozygous AG increase the risk of prostate cancer in carriers by 1.18 times (p-value =0.5, OR: 1.18, CI95%: 0.38- 3.61). But homozygous of wild genotype GG have a protective role against prostate cancer (p-value =0.005, OR: 0.385, CI95%: 0.23-0.65). Conclusion: Asn allele is one of the main factors in prostate cancer, therefore, it could be used as the non-invasive and suitable marker for early detection in susceptible individuals