Title of article
Molecular Detection of Zonula Occludens Toxin (zot) Genes in Vibrio Cholerae O1 using PCR
Author/Authors
Mousavi ، Mahnaz Department of Bioscience and Biotechnology - Malek Ashtar University of Technology , Zeinoddini ، Mehdi Department of Bioscience and Biotechnology - Malek Ashtar University of Technology , Azizi ، Azadeh Department of Bioscience and Biotechnology - Malek Ashtar University of Technology , Saeedinia ، Ali Reza Department of Bioscience and Biotechnology - Malek Ashtar University of Technology , Monazah ، Arina Department of Bioscience and Biotechnology - Malek Ashtar University of Technology
From page
37
To page
40
Abstract
Background: Zot (Zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that binds to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Methods: The genomic DNA was extracted by DNA isolation kit and gene amplification was carried out by the zot genespecific primers. Then, PCR products were investigated by electrophoresis on 1.2% agarose gel stained by ethidium bromide. Also, the specificity of primers was measured using bacterial samples other than V. cholerae, such as enterotoxigenic Escherichia coli )ETEC(, Salmonela. typhi and Aeromonas hydrophila. The sensitivity of the PCR reaction was also evaluated using serial dilutions of V. cholerae O1 concentration (cfu/ml). Results: The data showed that the designed primers specificity for zot gene was successful and the sensitivity of this method was determined about 142 cfu/ml. Conclusion: In conclusion, this molecular detection can be used as a simple diagnostic kit in clinical laboratories for identification of V. cholerae.
Keywords
Vibrio cholerae , zot , PCR , Diagnostic.
Journal title
Research in Molecular Medicine
Journal title
Research in Molecular Medicine
Record number
2507148
Link To Document