Evaluating of Iran Razi Khedmati Bovine Kidney (IRKHBK) Cell Line for Isolation and ‎Propagation of Viruses

Background and Aims: To identify and isolation of viruses a susceptible cell is needed, which can be primary or line. The use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. A cell line was originated from primary cells of bovine kidney by continuous passage. The aim of this study was to introduce this cell line for isolation, propagation and diagnosis of animal viruses. Materials and Methods: Samples of the infected animals, such as the lymph nodes, tonsils, lungs and blood were used for virus isolation. Primary BK cell and IRKHBK cell line cultures were inoculated with the prepared samples simultaneously under the same conditions. Identification of the isolated viruses was carried out with a virus neutralization test using standard antibodies. Results: Bovine Herpes Virus-1, Parainfluenza-3, Bovine viral diarrhea, and Peste des Petits Ruminants Virus were easily isolated and propagated in this cell line. The viral titer was higher in theses cells as compared to the primary BK cell. This cell line designated as IRKHBK (Iran Razi Khedmati Bovine kidney). Conclusion: The results show that this cell line can be used for virus isolation, detection and propagation. For both animal and human viruses for research and diagnostic tests.