Title of article :
Inhibition of Colorectal Cancer Cell Line CaCo-2 by Essential Oil of Eucalyptus camaldulensis Through Induction of Apoptosis
Author/Authors :
Taheri, Elham Tabriz University of Medical Sciences - Department of Pharmaceutical Biotechnology , Ghorbani, Saba Islamic Azad University, Tabriz Branch - Department of Genetics , Safi, Maryam Islamic Azad University, Tabriz Branch - Department of Genetics , Seyyed Sani, Nasrin Islamic Azad University, Tabriz Branch - Department of Genetics , Firouzi Amoodizaj, Fatemeh Islamic Azad University, Tabriz Branch - Department of Genetics , Heidari, Masoud University of Tabriz - Faculty of Natural Sciences - Department of Animal Biology , Chavoshi, Reza Islamic Azad University, Tabriz Branch - Department of Genetics , Hajazimian, Saba Tabriz University of Medical Sciences - Immunology Research Center , Isazadeh, Alireza Tabriz University of Medical Sciences - Immunology Research Center , Heidari, Mansour Tehran University of Medical Sciences - School of Medicine - Department of Medical Genetics
Abstract :
Treatment of colorectal cancer is one of the important challenges due to the increase of resistance to chemotherapeutic drugs. Isolated natural compounds from medicinal plants and other sources often are used as novel drugs for treatment of various human cancer. The aim of this study was to investigate the antioxidant and anticancer activity of Eucalyptus camaldulensis essential oil on colorectal cancer cell line Caco-2. The antioxidant activity of extracted E. camaldulensis essential oil (1000, 800, 400, 200, 100, 50, 25, 12.5, 6, and 3 μg/mL) was evaluated by free radicals inactivation method. Moreover, MTT assay was used to examine the cytotoxic effects of E. camaldulensis essential oil on the Caco-2 cell line. The mRNA expression of BAX and BCL-2 genes was studied using quantitative Real-Time PCR method, in treated cancer cells compared to untreated cells. We indicated a significant, impressive antioxidant activity in 1000 μg/mL of E. camaldulensis essential oil, in a concentration-dependent manner. In addition, we found that this product exerted a cytotoxic effect on cancer cells when 100 μg/mL concentration was considered as half-maximal inhibitory concentration (IC50). Also, the expression of BAX and BCL-2 genes were significantly upregulated and downregulated, respectively, in the treated Caco-2 cells with E. camaldulensis essential oil. In conclusion, our study showed significant antioxidant and anticancer activity in E. camaldulensis essential oil in a concentration and time-dependent manner, which may be due to the reduction of free radicals and induction of apoptosis process in colorectal cancer cells.
Keywords :
Colorectal cancer , Eucalyptus camaldulensis , Apoptosis , Antioxidant
Journal title :
Addiction and Health
