Title of article :
Methylation levels of lysozyme gene in rainbow trout (Oncorhynchus mykiss) fed by commercial immunogen probiotic
Author/Authors :
Choubkar ، Ali Department of Fisheries Sciences - Faculty of Natural Resources - University of Tehran , Farahmand ، Hamid Department of Fisheries Sciences - Faculty of Natural Resources - University of Tehran , Mirvaghefi ، Alireza Department of Fisheries Sciences - Faculty of Natural Resources - University of Tehran , Alizadeh ، Houshang Department of Agronomy and Plant Breeding - Faculty of Agricultural Sciences and Engineering - University of Tehran , Abed-Elmdoust ، Amirreza Department of Fisheries Sciences - Faculty of Natural Resources - University of Tehran
Abstract :
In the present study, we evaluated the effect of commercial immunogenic prebiotic on the rate of methylation of type-C gene. A total of 120 rainbow trout were divided into two treatments, including a control group and another one with 2.0% commercial prebiotic immunogen each in three replicates. On the first, 15 and 45 days, DNA of adrenal tissue was extracted and treated with bisulfite. Samples were amplified by polymerase chain reaction (PCR) and sequenced. Based on the results, there was no significant difference (P 0.05) between the 1st day (R), 15th day of control (C), 15th day of immunogen (I) and 40th day of control (CS). However, there was a significant difference (P 0.05) between the 45th day immunogenicity (IS) and other samples, i.e. the CpG islet methylation rate in the IS samples was lower leading to increase in the expression of the lysozyme gene.
Keywords :
Lysozyme , DNA methylation , Epigenetics , Immunity
Journal title :
International Journal of Aquatic Biology
Journal title :
International Journal of Aquatic Biology
