Title of article :
Evaluation of mRNA Expression Levels of cyp51A and mdr1, Candidate Genes for Voriconazole Resistance in Aspergillus flavus
Author/Authors :
Fattahi ، Azam Department of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Zaini ، Farideh Department of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Kordbacheh ، Parivash Department of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Rezaie ، Sasan Department of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Safara ، Mahin Department of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Fateh ، Roohollah Department of Microbiology and Immunology - Faculty of Medicine - Qom University of Medical Sciences , Farahyar ، Shirin Department of Medical Mycology and Parasitology - Faculty of Medicine - Iran University of Medical Sciences , Kanani ، Ali Department of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Heidari ، Mansour Department of Medical Genetics - Exprerimental Medicine Research Center - Tehran University of Medical Sciences
Abstract :
Background: Voriconazole Resistance (VRC-R) in Aspergillus flavus isolates impacts the management of aspergillosis, since azoles are the first choice for prophylaxis and therapy. However, to the best of our knowledge, the mechanisms underlying voriconazole resistance are poorly understood. Objectives: The present study was designed to evaluate mRNA expression levels of cyp51A and mdr1 genes in voriconazole resistant A. flavus by a Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique. Materials and Methods: Five A. flavus isolates with resistance to VRC were examined by a RT-PCR approach. Results: Four out of five isolates revealed cyp51A and mdr1 mRNA overexpression. Interestingly, the isolate, which was negative for cyp51A and mdr1 mRNA expression showed a high voriconazole Minimum Inhibitory Concentration (MIC). Furthermore, a computational-based analysis predicted that voriconazole resistance could be mediated through cooperation with a network protein interaction. Conclusions: Our experimental and in silico findings may provide new insight in the complex molecular pathways of drug resistance and also could assist design an efficient therapeutic strategy for aspergillosis treatment.
Keywords :
Drug Resistance , Minimum Inhibitory Concentration , cyp51A , mdr1
Journal title :
Jundishapur Journal of Microbiology (JJM)
Journal title :
Jundishapur Journal of Microbiology (JJM)
