Title of article
Comparison of Different Extracts of Nettle in Quercetin and Evaluation of its Antimicrobial Activity
Author/Authors
Taghvaei ، Mahsa Department of Biochemistry - Payame Noor University , Fazilati ، MohamMad Department of Biochemistry - Payame Noor University , Nazem ، Habibollah Department of Biochemistry - Payame Noor University , Habibollahi ، Saeed Department of Biochemistry - Payame Noor University , Bastani ، Faegh Department of Biochemistry - Payame Noor University
From page
572
To page
583
Abstract
Nettle (Urtica dioica L.) is one of the most well-known medicinal plants. It has been mostly used for medical purposes. This study summarizes extensive results on the antimicrobial effect of Urtica dioica L. The aim of this study was to investigate several extraction methods of nettle extract for more access to flavonoid materials especially quercetin. For this purpose, three methods (soaking, sonication, soxhlet) and three solvents (chloroform, methanol, deionized water) were used for extraction. Finally, the highest amount of quercetin determined by HPLC was sonication for 90 min with methanol solvent. The effect of this extract was investigated on several pathogenic microbes including Escherichia coli, Entrecoccus faecalis, Klebsiella pneumoniae, Acinetobacter calcoaceticus, Candida albicans. Antibiogram testing revealed that Escherichia coli and Candida albicans were resistant to nettle extract, Acinetobacter calcoaceticus and Klebsiella pneumonia, were sensitive and Enterococcus faecalis was semi-susceptible
Keywords
Nettle , Quercetin , HPLC , Antibiogram
Journal title
Chemical Methodologies
Journal title
Chemical Methodologies
Record number
2510880
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