Title of article :
Kit formulation of active pharmaceutical ingredient d,l-HMPAO as a brain perfusion diagnostic system
Author/Authors :
Davarpanah ، Mohammadreza Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company , Abbasi ، Hossein Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company , Nabati ، Mehdi Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company , Sabahnoo ، Hamideh Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company , Bodaghi-Namileh ، Vida Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company , Mazidi ، Mohammad Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company , Movahhed-Tazehkand ، Hossein Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company , Mohammadnejad-Mehrabani ، Hossein Synthesis and Molecular Simulation Laboratory, Chemistry Department - Pars Isotope Company
Abstract :
The purpose of this research study is to prepare exametazime (d,l-HMPAO) kit for labeling with 99m-technetium radionuclide as a brain perfusion diagnostic system. Firstly, the active pharmaceutical ingredient d,l-HMPAO was prepared in two steps with the purity of 99.29 %. Its molecular structure was characterized using elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) technique. In second step, the d,l-HMPAO kit was prepared using six different formulations and labeled by technetium-99m radionuclide. The high radiochemical yield was attributed to the high amount of SnCl2 and adding phosphate buffer. The animal studies were conducted on the three-month old male Wistar rats. The results of the biodistribution studies revealed that, the mean activity in brain of all rats was above 1% ID/g. This showed the high isomerism purity of the synthesized compound (d,l-HMPAO) and optimization of the suggested formulations.
Keywords :
Exametazime , HMPAO , Kit Formulation , Nuclear medicine , Radiopharmaceutical , Technetium , 99m radionuclide
Journal title :
Progress in Chemical and Biochemical Research
Journal title :
Progress in Chemical and Biochemical Research
