Vitamin E and Selenium Facilitate the Osteogenesis and Adipogenesis of the Human Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells

Background and Aims: Previous studies have shown that adiposederived mesenchymal stem/ stromal cells are one of the sources of mesenchymal stem cells (MSCs) with the capacity to differentiate into various mesodermal cell lineages. MSCs with cytokines secretion capability, which contributes to repair damaged tissues have gained wide credence for future cellbased therapeutic applications. In this study, the effect of the different dosages of vitamin E and Selenium was assessed on the stemness of the human adipose tissuederived MSCs (ADMSCs). Materials and Methods: Following 24 hours of cell treatments with different dosages of vitamin E and Selenium, MTT assay was used to assess the effect of them on cell proliferation. Moreover, the stemness of the ADMSCs was assessed using osteogenic and adipogenic induction medium supplemented with the different dosages of the vitamin E and Selenium. Finally, Alizarin red and Oilred O staining were performed to detect matrix mineralization and lipid droplet accumulation, respectively. Results: MTT data revealed that the optimal concentration for vitamin E and Selenium were 125 micro;M and 121 micro;M for the viability of the ADMSCs. Moreover, the effect of vitamin E and Selenium were assessed by osteogenic and adipogenic differentiation by optimal dosages obtained by MTT assay, respectively. Maximum mineralization and lipid droplet aggregation of the differentiated cells were detected at IC50 in comparison with the control group. Conclusions: These results suggest that different dosages of vitamin E and Selenium could have various impacts on the proliferation and differentiation induction of human ADMSCs.