Title of article :
Dynamic of miRNA-101a-3p and miRNA-200a during Induction of Osteoblast Differentiation in Adipose-derived Mesenchymal Stem Cells
Author/Authors :
Aslani ، Somayeh Department of Biochemistry and Clinical Laboratories - School of Medicine, Stem Cell Research Center - Tabriz University of Medical Sciences , Rahbarghazi ، Reza Department of Applied Cell Sciences - Tabriz University of Medical Sciences , Rahimzadeh ، Sevda Department of Biochemistry and Clinical Laboratories - School of Medicine - Tabriz University of Medical Sciences , Rajabi ، Hadi Department of Biochemistry and Clinical Laboratories - School of Medicine - Tabriz University of Medical Sciences , Abhari ، Alireza Department of Biochemistry and Clinical Laboratories - School of Medicine - Tabriz University of Medical Sciences , Sakhinia ، Ebrahim Department of Medical Genetics - School of Medicine - Tabriz University of Medical Sciences
Abstract :
miRNAs are known as the cellular phenomena regulators that exert their effects in post-transcriptional level. Recent studies highlight the role of miRNAs in mesenchymal stem cells differentiation into osteoblasts. The purpose of this study was to recognize the pattern of miRNA-101a-3p and miRNA-200a expression during osteoblastic differentiation of human adipose tissue-derived mesenchymal stem cells. The cells were incubated in osteoblastic differentiation medium for a period of 21 days. Alizarin red S staining was performed to confirm the successful differentiation of adipose-derived mesenchymal stem cells into osteoblast cells. The expression levels of miRNA-101a-3p and miRNA-200a were analyzed by real-time PCR during 0, 7, 14, and 21 days after differentiation induction. Data exhibited the increase of extracellular red color deposition which was evident at the end of the incubation period. The expression of miRNA-101a-3p and miRNA-200a was up regulated during adipose-derived mesenchymal stem cells trans-differentiation into osteoblast-like cells. These miRNAs could be potential novel biomarkers for monitoring successful differentiation of mesenchymal stem cells toward osteoblasts.
Keywords :
Adipose , derived , mesenchymal stem cells , osteoblast , like differentiation , miRNA , 101a , 3p , miRNA , 200a , transcription
Journal title :
International Journal of Molecular and Cellular Medicine(IJMCM)
Journal title :
International Journal of Molecular and Cellular Medicine(IJMCM)
