Elucidating the Anti-aging Ursolic Acid through Evaluation of SIRT1 and SIRT6 in the Mice Skeletal Muscle Tissue

The process of skeletal muscle aging is linked to the development of age-related diseases such as metabolic syndrome, diabetes, cancer and Alzheimer’s disease. Hence, to further investigate the effect of Ursolic Acid (UA) endorses previous studies on skeletal muscle rejuvenation, we tested whether UA alters two prominent anti-aging biomarkers such as SIRT1 and SIRT6 in skeletal muscle. UA is a triterpenoid compound widely found in natural plants and fruits, with known effects on skeletal muscle. To conduct this experiment, UA dissolved in Corn Oil (200 mg ml-1) was administrated (200 mg kg-1 IP injection) twice daily for 7 days to male aged-mice C57BL/6. Then, mice skeletal muscle tissues were isolated and proteins were examined through immunofluorescence microscopy. Our findings clearly illustrated that UA enhances SIRT1 (~3.5 folds) and SIRT6 (~4 folds) expression levels, p < 0.001. To the best of our knowledge, UA seems to imitate the effects of exercise by increasing the SIRT1 and SIRT6 proteins level in the skeletal muscle tissue. Finally, UA can be recommended as a superior candidate to improve the diseases associated with skeletal muscle aging.