Title of article :
Inhibitory Effects of Swietenia Mahagoni Seeds Extract on Α-Glucosidase and Α-Amylase
Author/Authors :
Norodin ، N. S.Md Department of Bioprocess and Polymer Engineering - Center of Lipids Engineering and Applied Research (CLEAR), Ibnu Sina Institute Scientific Industrial Research (Ibnu Sina ISIR), School of Chemical and Energy Engineering - Universiti Teknologi Malaysia , Salleh ، L.Md Department of Bioprocess and Polymer Engineering - Center of Lipids Engineering and Applied Research (CLEAR), Ibnu Sina Institute Scientific Industrial Research (Ibnu Sina ISIR), School of Chemical and Energy Engineering - Universiti Teknologi Malaysia , Yusof ، N Advanced Membrane Technology Research Centre (AMTEC) - Universiti Teknologi Malaysia , Mustapha ، N. M Natural Product Division - Forest Research Institute Malaysia (FRIM) , Kamarulzaman ، F Natural Product Division - Forest Research Institute Malaysia (FRIM) , Zahari ، M. A.Mohamed Department of Bioprocess and Polymer Engineering - Center of Lipids Engineering and Applied Research (CLEAR), Ibnu Sina Institute Scientific Industrial Research (Ibnu Sina ISIR), School of Chemical and Energy Engineering - Universiti Teknologi Malaysia , Bakeri ، N. A Department of Bioprocess and Polymer Engineering - Center of Lipids Engineering and Applied Research (CLEAR), Ibnu Sina Institute Scientific Industrial Research (Ibnu Sina ISIR), School of Chemical and Energy Engineering - Universiti Teknologi Malaysia
Abstract :
This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays. Swietenia mahagoni seeds were extracted by using supercritical carbon dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C. The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays. All data obtained were expressed as mean ± standard deviation for triplicate experiments. One way analysis was used for statistical significance by using statistica software version 7.0 (StartSoft, EUA) and IC50 (extract concentration causing 50% enzyme inhibitory) was determined by using GraphPad Prism 6.0 software. Swietenia mahagoni seeds extract have a strong inhibition of α-glucosidase enzyme activity (98.4% ± 0.2) but a moderate inhibition of α- amylase enzyme activity (34.9% ± 1.2). These findings implied that Swietenia mahagoni seeds extract could be an effective natural antidiabetic agent.
Keywords :
Swietenia Mahagoni Seed , Α , Glucosidase Enzyme , Α , Amylase Enzyme , Supercritical Carbon Dioxide (SC , CO2) Extraction
Journal title :
International Journal of Engineering
Journal title :
International Journal of Engineering
