Title of article :
Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats
Author/Authors :
Ziaei, Atousa Traditional Medicine and Material Medical Research Center - Department of Traditional Pharmacy - School of Traditional Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Sahranavard, Shamim Traditional Medicine and Material Medical Research Center - Department of Traditional Pharmacy - School of Traditional Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Gharagozlou, Mohammad Javad Department of Pathology - Faculty of Veterinary Medicine - University of Tehran , Faizi, Mehrdad Department of Pharmacology and Toxicology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Background: Many plants have been introduced in Iranian traditional medicine for treatment of different joint problems including knee pain. Topical application of the mixture of Lawsonia inermis L. leaves (Henna) with
aqueous extract of Ricinus communis L. leaves have been mentioned to have significant effects on reducing knee
pain. The present study was designed to evaluate the analgesic and anti-inflammatory effects of the mixture of
these two herbs in male rats.
Methods: We induced knee osteoarthritis as a model of chronic pain by intra-articular injection of mono sodium
iodoacetate (MIA). Mechanical allodynia, hotplate latency test, spontaneous movements and gait analysis were used
for the evaluation of analgesic activity. Anti-inflammatory activity was evaluated by measuring the diameter and the
volume of the injected paw compared to contralateral paw. These tests were monitored at days 1, 3, 7, 14 and 21 of
MIA administration. Histopathological evaluations were also used to assess the efficacy of the treatment on
inflammation and lesions in knee tissue. In all tests, diclofenac topical gel was used as a positive control. The herbal
extracts, their mixture, and vehicle or diclofenac gel were administered daily for 14 days by topical route.
Results: The mixture of these two extracts significantly reduced the knee joint width and volume of the injected paws
and also improved foot prints in gait analysis after 3 days of MIA injection. Analysis of mechanical allodynia (after
21 days), hotplate latency test (after 10 days), spontaneous movements (after 7 days) and in positive control group
(after 3 days in all tests and in mechanical allodynia after 14 days) compared to the vehicle group, showed significant
effects. Topical usage of the selected formulation made significant histopathological changes on the knee of the rats.
Compared to the vehicle group, the tests and diclofenac groups showed less reactions characterized by negligible
edema and a few scattered inflammatory lymphoid cells.
Conclusion: The present findings showed that the present formulation not only was able to mitigate pain
and inflammation in the paws but also made significant histopathological changes on the knee of the rats. Further studies are necessary to confirm the effect of the formulation.
Keywords :
Lawsonia inermis L , Leaves , MIA , Osteoarthritis , Rats , Ricinus communis L
Journal title :
Daru:Journal of Pharmaceutical Sciences