Title of article :
Spectroscopic analysis of bosentan in biological samples after a liquid-liquid microextraction
Author/Authors :
Sajedi-Amin Sanaz Department of Analytical Chemistry - Faculty of Chemistry - University of Tabriz - Tabriz, Iran , Assadpour-Zeynali Karim Department of Analytical Chemistry - Faculty of Chemistry - University of Tabriz - Tabriz, Iran , Panahi-Azar Vahid Drug Applied Research Center - Tabriz University of Medical Sciences - Tabriz, Iran , Kebriaeezadeh Abbas Pharmaceutical Administration Research Center - Tehran University of Medical Sciences - Tehran, Iran , Khoubnasabjafari Maryam Tuberculosis and Lung Disease Research Center - Tabriz University of Medical Sciences - Tabriz, Iran , Ansarin Khalil Tuberculosis and Lung Disease Research Center - Tabriz University of Medical Sciences - Tabriz, Iran , Jouyban-Gharamaleki Vahid Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company - Tabriz University of Medical Sciences - Tabriz, Iran , Jouyban Abolghasem Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company - Tabriz University of Medical Sciences - Tabriz, Iran
Abstract :
Microextraction processes with UV-Vis
measurement have been developed and validated for analysis
of bosentan in biological samples.
Methods: In this work, liquid–liquid microextraction
procedures (DLLME & USAEME) were employed for
cleanup, pre-concentration, and determination of bosentan
in biological samples by UV-Vis spectroscopy at 270 nm.
The method was validated and applied to the determination
of bosentan in spiked serum, exhaled breath condensate and
urine samples.
Results: Various experimental factors including type of extraction and dispersive solvents
and their volumes, pH, sonication time and centrifuging time were investigated. Under the
optimum conditions, the method was linear in the range of 1.0–5.0 μg.mL−1, with coefficient of
determination (R2) of > 0.998. The limit of detection (LOD) was 0.07 mg.L−1. Recovery of the
target analyte in biological samples was 106.2%. The method could be easily applied for higher
concentration of bosentan and needs more improvement for application in the pharmacokinetic
investigations where more sensitive methods are required.
Conclusion: A simple, low cost, precise and accurate spectrophotometric analysis of bosentan in
biological samples after liquid-liquid microextraction were developed and validated for routine
analyses.
Keywords :
Liquid–liquid microextraction , Exhaled breath condensate , Serum , Urine samples
Journal title :
Bioimpacts
