Title of article :
Evaluation of miR-182/miR-100 Ratio for Diagnosis and Survival Prediction in Bladder Cancer
Author/Authors :
Chen, Zhanguo OLDWHG Hospital of Wenzhou Medical University - China , Wu, Lili Department of Clinical Laboratory - The Dingli Clinical College of Wenzhou Medical University - China , Lin, Qi School of the First Clinical Medical Sciences - Wenzhou Medical University - China , Shi, Jing Department of Human Anatomy - The Fenyang College of Shanxi Medical University -China , Lin, Xiangyang OLDWHG Hospital of Wenzhou Medical University - China , Shi, Liang OLDWHG Hospital of Wenzhou Medical University - China
Abstract :
Background
Abnormal expression of microRNAs (miRNAs) plays an important role in development of several cancer types, including bladder cancer (BCa). However, the relationship between the ratio of miR-181/miR-100 and the prognosis of BCa has not been studied yet. The aim of this study was to evaluate the expression of miR-182, miR-100 and their clinical significance in BCa.
Methods
Upregulation of miR-182 and down-regulation of miR-100 were validated in tissue specimens of 134 BCa cases compared with 148 normal bladder epithelia (NBE) specimens using TaqMan-based real-time reverse transcription quantitative PCR (RT-qPCR). The diagnostic and prognostic evaluation of miR-182, miR-100, and miR-182/miR-100 ratio was also performed.
Results
miR-182 was upregulated in BCa and miR-100 was down-regulated in BCa compared with NBE (P
Conclusion
The miR-182/miR-100 ratio may serve as a novel promising biomarker for diagnosis and survival prediction in BCa. Further studies are needed to elucidate the role of miR-182/miR-100 ratio as a non‑invasive diagnostic tool for BCa.
Keywords:
Keywords :
Biomarker , bladder cancer , miR , 182 , miR , 100 , prognosis
Journal title :
Archives of Iranian Medicine
