Title of article :
Dysbiosis of fecal microbiota and high frequency ofCitrobacter,Klebsiellaspp., and Actinomycetes in patients with irritable bowel syndrome and gastroenteritis
Author/Authors :
Ganji, Leila Department of Pathobiology - School of Public Health - University of Medical Sciences, Tehran, Iran , Alebouyeh, Masoud Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Science, Tehran, Iran , Shirazi, Mohammad Hassan Department of Pathobiology - School of Public Health - University of Medical Sciences, Tehran, Iran , Eshraghi, Saeed Department of Pathobiology - School of Public Health - University of Medical Sciences, Tehran, Iran , Mirshafiey, Abbas Department of Pathobiology - School of Public Health - University of Medical Sciences, Tehran, Iran , Ebrahimi Daryani, Naser Department of Gastroenterology and Hepatology - School of Medicine - Tehran University of Medical Sciences, Iran , Zali, Mohammad Reza Department of Pathobiology - School of Public Health - University of Medical Sciences, Tehran, Iran
Abstract :
Aim: This study was aimed to characterize putative differences of fecal microbiota between irritable bowel syndrome
(IBS) and gastroenteritis patients and healthy controls.
Background: New evidence proposed that gut microbiota has a deep effect on the balance between health and disease.
Patients and methods: The presence of Clostridium difficile, Campylobacter spp., Enterobacteriacea and Staphylococci were
detected in the samples using selective and specific culture media. Microscopic examination of the samples was done to detect
Actinomycetes, yeasts, Bifidobacteria, Fusobacterium spp., as well as white blood cells, red blood cells, mucus and epithelial cells.
Results: Results of this study showed relatively higher frequency of Citrobacter spp., Lactobacilli, and Actinomycetes in the
IBS patients. Elevated levels of WBC, RBC secretion, and increased amounts of Klebsiella, Escherichia coli and Citrobacter
spp. were characterized in the patients with gastroenteritis compared with the control group.
Conclusion: Depletion of gram positive cocci and gram negative bacilli also suggested dysbiosis of intestinal microbiota
in these patients.
