Author/Authors :
Valadan Tahbaz, Sahel Department of Microbiology - Faculty of Basic Sciences - Islamic Azad University Research and Science Branch, Tehran, Iran , Yadegar, Abbas Foodborne and Waterborne Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Amirmozafari, Nour Microbiology Department - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Yaghoobee, Siamak Department of Periodontics and Implant - Faculty of Dentistry - Tehran University of Medical Sciences, Iran , Ehsani Ardakani, Mohammad Javad Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Zojaji, Homayoun Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Aim: This study was aimed to investigate the presence of H. pylori and its virulence genotypes in dental plaques of Iranian patients
with chronic periodontitis.
Background: Helicobacter pylori is a Gram-negative bacterium that is associated with atrophic gastritis, peptic ulcer, and gastric
cancer. Several studies have detected this bacterium in the oral cavity, suggesting it as a potential reservoir.
Methods: A hundred individuals were divided in 2 groups: 50 patients with chronic periodontitis (case group), and 50 subjects in
non-periodontitis (control group). Supragingival and subgingival plaque samples were collected from the individuals using wood
wedges and sterile paper points respectively, and prepared for PCR analysis.
Results: Totally, H. pylori DNA was detected in 5 out of 100 (5%) dental plaques. Of 5 dental plaques positive for H. pylori, cagA
gene was detected in 4 specimen, 3 in periodontitis group and one in non-periodontitis group. The H. pylori vacA s1m1 genotype was
predominantly detected in 2/5 samples. The babA2 gene was detected in all (5/5) H. pylori-positive dental plaques. There was no
significant correlation between the presence of H. pylori genotypes from dental plaques and chronic periodontitis (P > 0.05).
Conclusion: Our results revealed that the rate of H. pylori is very low in the dental plaques of Iranian patients with chronic periodontitis.
Majority of H. pylori strains from oral cavity were highly virulent based on the main clinically virulence factors they carried.
Keywords :
Helicobacter pylori , Chronic periodontitis , Virulence genotypes , Dental plaque , PCR
