Author/Authors :
Zarandi, Ashkan Department of Biology - Islamic Azad University Science and Research Branch Tehran, Iran , Irani, Shiva Department of Biology - Islamic Azad University Science and Research Branch Tehran, Iran , Savabkar, Sanaz Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Chaleshi, Vahid Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ghavideldarestani, Maryam Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ghavideldarestani, Maryam Department of Medical Genetics - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Khodadoostan, Mahsa Department of Gastroenterology and Hepatology - Isfahan University of Medical Sciences, Iran , Nazemalhosseini-Mojarad, Ehsan Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Asadzadeh Aghdaei, Hamid Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Aim: The aim of this study was to evaluate the methylation status of the promoter region of MLH1 gene in colorectal cancer (CRC)
and its precursor lesions as well as elucidate its association with various clinicopathological characteristics among Iranian population.
Background: Epigenetic silencing of mismatch repair genes, such as MLH1, by methylation of CpG islands of their promoter region has
been proved to be an important mechanism in colorectal carcinogenesis.
Methods: Fifty colorectal cancer and polyp tissue samples including 13 Primary colorectal tumor and 37 Adenoma polyp samples
were enrolled in this study. Methylation-specific polymerase chain reaction (MSP) was performed to find the frequency of
MLH1 Promoter Methylation.
Results: Promoter methylation of MLH1 gene was detected in 5 out of 13 tumor tissues and 4 out of 37 adenoma polyp. The
frequency of MLH1 methylation in tumor samples was significantly higher compared to that in polyp tissues (P= 0.026). No
significant association was observed between MLH1 promoter methylation and clinicopathological characteristics of the patients.
Conclusion: The frequency of MLH1 promoter methylation in CRC and colon polyp was 18%. Our findings indicated that
methylation of MLH1 promoter region alone cannot be considered as a biomarker for early detection of CRC.
Keywords :
Colorectal polyp , MLH1 , Promoter methylation , MSP
