Author/Authors :
Abbaszadegan, Abbas Department of Endodontics - Dental School - Shiraz University of Medical Sciences, Shiraz , Gholami, Ahmad Pharmaceutical Sciences Research Center - Shiraz University of Medical Sciences , Ghahramani, Yasamin Department of Endodontics - Dental School - Shiraz University of Medical Sciences, Shiraz , Ghareghan, Razieh Students' Research Committee - Dental School - Shiraz University of Medical Sciences, Shiraz , Ghareghan, Marzieh Students' Research Committee - Dental School - Shiraz University of Medical Sciences, Shiraz , Kazemi, Aboozar Pharmaceutical Sciences Research Center - Shiraz University of Medical Sciences , Iraji, Aida Central Laboratory - Shiraz University of Medical Science, Shiraz , Ghasemi, Younes Pharmaceutical Sciences Research Center - Shiraz University of Medical Sciences
Abstract :
Introduction: The aims of this study were i) to define the chemical constituents of Cuminum cyminum (cumin) essential oil, ii) to compare the antimicrobial activity of this oil to that of chlorhexidine (CHX) and co-trimoxazole on planktonic and biofilm forms of bacteria isolated
from the teeth with persistent endodontic infection and iii) to compare the cytotoxicity of
these medicaments on L929 fibroblasts. Methods and Materials: Three groups of
microorganisms [aerobic bacterial mixture, anaerobic bacterial mixture and Enterococcus
faecalis (E .faecalis)] were isolated from the teeth with persistent apical periodontitis. Zone of
inhibition (ZOI), minimum inhibitory concentration (MIC), minimum bactericidal
concentration (MBC), minimum biofilm inhibitory concentration (MBIC) and time-kill tests
were performed to assess the antimicrobial efficacy of the medicaments. Further, a
cytocompatibility analysis of the medicaments was performed on L929 fibroblasts. The results
obtained from disc diffusion test and mean cell viability values of the experimental
medicaments were analyzed using two-way and one-way analysis of variance (ANOVA).
Results: Seventeen constituents were recognized in cumin oil (predominantly cumin
aldehyde and γ-terpinene). Co-trimoxazole showed the greatest ZOI followed by cumin and
CHX. The smallest MIC and MBC belonged to co-trimoxazole followed by cumin and CHX
for all groups of bacteria except for E. faecalis for which the MBC of cumin was smaller than
co-trimoxazole. The results of time-kill assay revealed that all medicaments totally inhibited
the bacterial growth in all groups after 24 h. CHX was the most cytotoxic solution while
there were no significant differences between the cytocompatibility of different
concentrations of cumin essential oil and co-trimoxazole. Conclusion: Cumin exhibited a strong antimicrobial efficiency against the microbial flora of the teeth with failed endodontic treatments and it was biocompatible for L929 mouse fibroblasts.
Keywords :
Antibacterial Activity , Chlorhexidine , Co-trimoxazole , Cuminum Cyminum , Cytotoxicity , Intracanal Medicament
