Author/Authors :
Nasiri, Saleh Department of Biotechnology - Blood Transfusion Research Center - High Institute for Research and Education in Transfusion Medicine, Tehran , Abbasi, Fatemeh Department of Production - Tehran Blood transfusion Center, Tehran
Abstract :
Background: Platelets rapidly lose their qualities usually after 5 day of storage.
Different standard methods have been recommended to check the quality of
platelets during storage which some of them show better correlation with other
quality markers during storage. The purpose of this study was to demonstrate if
platelet factor 3 (PF3) assay could be an indicator of storage lesion and provide
a significant correlation with other quality markers during long-term storage of
platelet concentrates (PC) up to 11 days.
Methods: Twelve random units of PC were placed in a standard platelet incubator
under continuous agitation at 22-24°C for eleven days. Samples were taken on
days 1, 3, 5, 8 and 11. Parameters such as pH, glucose, lactate dehydrogenase
(LDH), platelet count of the bags, mean platelet volume (MPV) and platelet
distribution width (PDW) and PF3 were measured. The correlation coefficient of
PF3 and pH with the abovementioned parameters was evaluated.
Results: The mean percentage of changes for PF3, pH, glucose, LDH, platelet
count, MPV and PDW on day 11 compared to the first day were found to be 61,
15, 52, 440, 19, 18 and 39%, respectively. After LDH, PF3 had the highest change
relative to the other markers. PF3 demonstrated better correlation with glucose,
platelet count, MPV and PDW compared with pH during long-term PC storage.
Conclusion: Platelet factor 3 based-clotting time assay could be a potential
candidate for monitoring the quality of PC due to apparent trend of its changes
during storage with better correlation between the quality markers.
Keywords :
Platelet concentrate , Platelet factor 3 , pH , LDH , MPV , Platelet storage lesions , Quality marker , Platelet recovery
