Author/Authors :
Guan, Jingjing Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Wang, Zhongyong Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Liu, Xiaoyuan School of Laboratory Medicine and Life Sciences - Wenzhou Medical University, Wenzhou , Jiang, Yujie Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Gao, Qiuqi Department of Intensive Care Unit - The First Affiliated Hospital of Wenzhou Medical University - Wenzhou, China , Wu, Qing Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Lu, Hong Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Wu, Lianfeng Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Zhang, Zhuo Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Lin, Xiangyang Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou , Qian, Jingjing Department of Clinical Laboratory - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou
Abstract :
Background: Given the high mortality of bacterial bloodstream infections (BSI), blood
culture results do not meet clinical needs timely due to being time-consuming and
having low positive rate. Whether we can identify the severity and type of bacterial
infections by cytokines is a controversial issue. Objective: To investigate the dynamic
change of cytokines in BSI. Methods: 55 patients with Gram-positive (GP) BSI, 64
patients with Gram-negative (GN) BSI and 52 healthy controls were enrolled. We
quantitatively detected the cytokines interleukin (IL)-2, IL-4, IL-6, IL-10, tumor
necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) by flow cytometry in the
sera. The levels of procalcitonin, C-reactive protein, leukocytes and neutrophils were
also detected simultaneously. Results: There were significantly up-regulated IL-6 and
IL-10 expression in BSI patients, particularly in the GN-BSI, for instance Escherichia
coli and Klebsiella pneumoniae infections; following the treatment, IL-6 and IL-10
decreased by 10-23 and 4-27 times, respectively. Additionally, IL-2, TNF-α and IFN-γ
expression increased slightly in BSI patients and IFN-γ expression declined as GN-BSI
progressed. Conclusion: IL-6 and IL-10 are closely associated with the severity and
treatment efficacy of BSI, and can help to distinguish between GP-BSI and GN-BSI at
an early stage.
Keywords :
Bloodstream Infection , Cytokines , Gram-Negative Bacteria , IL-6 , IL-10
