Author/Authors :
SIYADATPANAH, Abolghasem Ferdows School of Paramedical and Health - Birjand University of Medical Sciences, Birjand, Iran , DARYANI, Ahmad Department of Parasitology - Toxoplasmosis Research Center - Mazandaran University of Medical Sciences, Sari, Iran , SARVI, Shahabeddin Department of Parasitology - Toxoplasmosis Research Center - Mazandaran University of Medical Sciences, Sari, Iran , SPOTIN, Adel Immunology Research Center - Tabriz University of Medical Sciences, Tabriz, Iran , SHARIF, Mehdi Department of Parasitology - Toxoplasmosis Research Center - Mazandaran University of Medical Sciences, Sari, Iran , ESMAEELZADEH DIZAJI, Reza Department of Poultry Disease - Faculty of Veterinary Medicine - University of Tehran, Tehran, Iran , ANVARI, Davood Department of Parasitology - Toxoplasmosis Research Center - Mazandaran University of Medical Sciences, Sari, Iran , EMAMI ZEYDI, Amir Department of Medical-Surgical Nursing - Nasibeh School of Nursing and Midwifery - Mazandaran University of Medical Sciences, Sari, Iran , KOHANSAL, Mohammad Hasan School of Medicine - Bam University of Medical Sciences, Bam, Iran , AGUDELO HIGUITA, Nelson Ivan Division of Infectious Diseases Department of Medicine - University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA , HOSSEINI, Abdollah Department of Parasitology - Toxoplasmosis Research Center - Mazandaran University of Medical Sciences, Sari, Iran , ARYAEIPOUR, Mojgan Department of Medical Parasitology and Mycology - School of Public Health -Tehran University of Medical Sciences, Tehran, Iran , GHOLAMI, Shirzad Department of Parasitology - Toxoplasmosis Research Center - Mazandaran University of Medical Sciences, Sari, Iran
Abstract :
Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinococcus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics.
Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity.
Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified.
Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.
