Title of article :
Hypericin induces apoptosis in AGS cell line with no significant effect on normal cells
Author/Authors :
Naderi, Misagh ntegrative Oncology Department - Breast Cancer Research Center - Motamed Cancer Institute - ACECR, Tehran, Iran , Rahmani Cherati, Mahtab Department of Biology - Science and Research Branch - Islamic Azad University, Tehran, Iran , Mohammadian, Ali Department of Medical Biotechnology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran, Iran , Bidhendy, Mohammad Baghery Clinical Research Development Center - Amir-almomenin Hospital - Tehran Medical Sciences Branch - Islamic Azad University, Tehran, Iran , Ghiasvand, Saeedeh Departments of Biology - Faculty of Science - Malayer University, Malayer, Iran , Zare Marzouni, Hadi Student Research Committee - Mashhad University of Medical Sciences, Mashhad, Iran , Aryan, Hoda Young Researchers and Elite Club - Tehran Medical Sciences Branch - Islamic Azad University, Tehran, Iran , Jangholi, Ehsan Young Researchers and Elite Club - Islamic Azad University - Tehran Medical Sciences Branch, Tehran, Iran , Javidi, Mohammad Amin ntegrative Oncology Department - Breast Cancer Research Center - Motamed Cancer Institute - ACECR, Tehran, Iran
Pages :
9
From page :
349
To page :
357
Abstract :
Introduction: It is of great importance to find an effective approach that not only eliminates gastric cancer cells but also do exhibits significant side effect to normal cells. Some studies have shown the effectiveness of hypericin against cancer cells. In this study, we evaluated the anti-cancer effect of Hypericin in the treatment of gastric cancer. Methods: In this study, the AGS cell line was exposed to different concentrations of hypericin for 24 and 48 hours. Evaluation of cell death was done by MTT assay. The rate of apoptosis was measured by flow cytometry assay using Annexin V / Propidium Iodide. The expression rate of Bcl2, p53 and Bax genes was evaluated by Real-time PCR test, and immunocytochemistry (ICC) analysis and western blotting was used for further evaluation of P53. Results: MTT assay test showed that hyepricin induces 50% cell death in the concentration of 1(µg/ml) and 0.5(µg/ml) at 24 h and 48 h post-treatment, respectively, however no similar effect seen on fibroblast cells. Annexin/PI test revealed that cell apoptosis after exposure to hypericin for 24 hours was 74%. Real-time PCR showed that expression level of Bax, p53 and Bax genes increases and Bcl2 gene decreases in AGS cell lines after treatment by hypericin. ICC analysis and western blotting for p53 confirmed these data. Conclusion: The results of this study indicated that hypericin has the potential to be introduced as an effective treatment for gastric cancer. Therefore, it seems that this substance has potential to be utilized as anti-cancer drug.
Keywords :
Hypericin , apoptosis , AGS , anticancer drug , gastric cancer , herbal medicine
Journal title :
Iranian Journal of Pharmaceutical Research(IJPR)
Serial Year :
2020
Record number :
2519981
Link To Document :
بازگشت