Title of article :
Tanacetum parthenium enhances pentobarbital-induced sleeping behaviors
Author/Authors :
Forouzanfar ، Fatemeh Department of Neuroscience - Neuroscience Research Center, Faculty of Medicine - Mashhad University of Medical Sciences , Ghazavi ، Hamed Department for Population and Family Health, Vice Chancellery for Health - Mashhad University of Medical Sciences , Vahedi ، Mohammad Mahdi Department of Pharmacology - Health Promotion Research Center, School of Medicine - Zahedan University of Medical Science , Tarah ، Kimia Pharmacological Research Center of Medicinal Plants - Mashhad University of Medical Sciences , Yavari ، Zahra Medical Toxicology Research Center, School of Medicine - Mashhad University of Medical Sciences , Hosseini ، Azar Pharmacological Research Center of Medicinal Plants - Mashhad University of Medical Sciences , Aghaee ، Azita Pharmacological Research Center of Medicinal Plants - Mashhad University of Medical Sciences , Rakhshandeh ، Hasan Pharmacological Research Center of Medicinal Plants - Mashhad University of Medical Sciences
Abstract :
Objective: Sleep disorders are among the most common psychiatric and medical conditions. In the present study, the hypnotic effect of Tanacetum parthenium was studied in mice. Materials and Methods: The hydro-alcoholic extract (HAE) of T. parthenium and three fractions of it, namely water fraction (WF), ethyl acetate fraction (EAF), and n-hexane fraction (NHF), were intraperitoneally (ip) administrated to mice 30 min before injection of sodium pentobarbital (30 mg/kg, ip). Then, 30 min after administration of HAE, motor coordination (rota-rod test) was evaluated. Besides, LD50 of HAE was determined and the cytotoxicity of HAE was evaluated in PC12 cells using the MTT assay. Results: HAE 50-200 mg/kg increased the sleeping time. EAF was the only fraction which could prolong the sleep duration and decrease sleep latency. The LD50 value was 4.8 g/kg. The extract induced no cytotoxic effects in PC12 cell line. Conclusion: The results suggested that T. parthenium potentiates pentobarbital hypnosis without causing toxic effects. Probably, its effects are mediated by the components present in EAF of this plant.
Keywords :
Pentobarbital , PC12 , Sleep , Tanacetum parthenium , Herbal Medicine
Journal title :
Avicenna Journal of Phytomedicine AJP)
Journal title :
Avicenna Journal of Phytomedicine AJP)
