Title of article :
Evaluation antibacterial activity of Biosynthesized Silver Nanoparticles using e Euphorbia Pseudocactus Berger extracts (Euphorbiaceae)
Author/Authors :
Sadeghipour, Yasin Department of Medical Nanotechnology - School of Advanced Medical Sciences and Technologies - Shiraz University of Medical Sciences , Alipour, Mohammad Hassan Social Security Organization,shahid beheshti hospital, shiraz , Ghaderi Jafarbeigloo, Hamid Reza Department of Basic Science - Payame Noor University , Salahvarzi, Afsaneh Department of Chemistry and Nanochemistry - Faculty of Sciences & Modern Technologies , Mirzaii, Mehdi Department of Medical Microbiology - School of Medicine - Shahroud University of Medical Sciences , Amani, Ali mohammad Pharmaceutical Sciences Research Center - Shiraz University of Medical Sciences - Department of Medical Nanotechnology - School of Advanced Medical Sciences and Technologies - Shiraz University of Medical Sciences , Mosleh-shirazi, Sareh Department of Material Engineering - Shiraz University of Technology, Shiraz , Mehrabi, Mohsen Department of Medical Nanotechnology - School of Medicine - Shahroud University of Medical Sciences
Abstract :
In the current research, silver nanoparticles (Ag NPs) were created usingEuphorbia
Pseudocactus Berger (Euphorbiaceae) extracts, which played the main role in
the formation and stability of nanoparticles. The physic-chemical properties
of biosynthesized nanoparticles were characterized by means of Ultraviolet–
Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), and Transmission Electron
Microscopy (TEM), and Fourier-Transform Infrared spectroscopy (FT-IR) methods.
UV-Vis results illustrated that maximum plasma resonance absorption of Ag NPs
are about 426 nm. Size distribution and spherical morphology was determined
by TEM method. The XRD was confirmed face centered cubic (FCC) structure
for synthesized nanoparticles. Monte Carlo (MC) simulations and Molecular
dynamics (MD) were utilized to evaluate the nanoparticles. The antibacterial
properties of biosynthesized Ag NPs were studied on E. coli (ATCC 25922), S.
aureus (ATCC 2592), P. aeruginosa (ATCC27853) and E. faecalis (ATCC51299)
using micro dilution broth method. The minimum inhibitory concentration
(MIC) results of synthesized Ag NPs on S. aureus and E. faecalis obtained 4 and
8 μg/mL and P. aeruginosa and E. coli obtained 16 and 4 μg/mL. So, synthesized
nanoparticles can be utilized as an antibacterial agent in medical and industrial
devices and tools.
Keywords :
silver nanoparticles , Euphorbia Pseudocactus Berger (Euphorbiaceae) , antibacterial , molecular dynamics
Journal title :
Nanomedicine Research Journal
