Green synthesis Zinc Oxide nanoparticle using Allamanda cathartica leaf extract and their cytotoxic and antibacterial activity

The objective of this study is to investigate the in vitro antibacterial and cytotoxicity activities of green synthesized zinc oxide (ZnO) nanoparticles using aqueous leaf extract of Allamanda cathartica (L). Zinc nitrate serves as a precursor while the aqueous leaf extract as chemical reducing agent. Green synthesized ZnO nanoparticles are confirmed by an analysis of the powder X-ray diffraction. The FTIR analysis indicates the existence of various functional groups in both the leaf extract and the Zno nanoparticles. The wavelength of the UV absorption was measured to a maximum of 360 nm. ZnO nanoparticles are measured for its crystalline form, shape and surface morphology using Field Emission Scanning Electron Microscopy (FE-SEM). The EDAX spectrum confirms that the ZnO nanoparticles contains zinc and oxygen. Green synthesized ZnO nanoparticles demonstrated significant antibacterial activity against clinical bacillus sp. The anticancer acvitiy of ZnO nanoparticles were studied against human breast cancer MCF7 cell and the proliferation of MCF7 cell was substantially reduced when compared with the control cell viability