Effect of Endurance Training with Coriander Seed Consumption on Caspase-3 and Cytochrome-C in the Heart Tissue of H2O2-Poisoned Rats

Background: Oxidative stress is a process created by free radicals at the cellular level and can lead to structural damage in different parts of the cell. However, training (T) and medicinal plants with antioxidant effects can prevent these damages. Objectives: The aim of present study was to investigate the effect of T with coriander seed (CS) consumption on caspase-3 and cytochrome-C in the heart tissue of hydrogen peroxide (H2O2)-poisoned rats. Methods: In this experimental study, 35 male Wistar rats were divided into 7 groups of 5 rats, including: 1) healthy control, 2) sham, 3) T, 4) 500 mg/kg CS, 5) 1000 mg/kg CS, 6) T+500 mg/kg CS, and 7) T+1000 mg/kg CS. During eight weeks, groups 2 - 7 received 1 mmol/kg H2O2 peritoneally for three times per week; groups 4 - 7 received CS at given doses. Also, groups 3, 6 and 7 performed T three days per week. Data analysis was performed using independent sample t-test, two-way analysis of variance and Bonferroni’s post-hoc tests. Significance level was set at 0.05. Results: H2O2 poisoning significantly increased caspase-3 and cytochrome-C compared to the sham group (P = 0.001); T and CS significantly decreased caspase-3 and cytochrome-C (P = 0.001), and interactive effects of T and CS were significant compared to the sham group (P ≥ 0.05). In addition, 1000 mg/kg CS had more effect on decrease of caspase-3 and cytochrome-C than 500 mg/kg CS (P ≥ 0.05). Discussion: T and CS consumption appear to have interactive effects on decrease of caspase-3 and cytochrome-C in the heart tissue of rats poisoned with H2O2; also effects of CS can be dose-dependent.