Author/Authors :
Salehi, Majid Department of Tissue Engineering - School of Medicine - Shahroud University of Medical Sciences, Shahroud, Iran , Ai, Arman School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Ehterami, Arian Department of Mechanical and Aerospace Engineering - Islamic Azad University - Science and Research Branch, Tehran, Iran , Einabadi, Masoumeh Department of Biology - Islamic Azad University - Jahrom Branch, Jahrom, Iran , Taslimi, Alireza Dentistry Student - Scientific Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Ai, Armin Dentistry Student - Scientific Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Akbarzadeh, Hamta Dentistry Student - Scientific Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Jabal Ameli, Ghazal Dentistry Student - Scientific Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Farzamfar, Saeed Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran , Shirian, Sadegh Department of Pathology - School of Veterinary Medicine - Shahrekord University, Shahrekord, Iran , Azimi, Nahal Dentistry Student - Scientific Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Sadeghi, Faezeh Dentistry Student - Scientific Research Center - School of Dentistry - Tehran University of Medical Sciences, Tehran, Iran , Bahrami, Naghmeh Craniomaxillofacial Research Center - Tehran University of Medical Sciences, Tehran, Iran , goodarzi, Arash Department of Tissue Engineering - School of Advanced Technologies in Medicine - Fasa University of Medical Sciences, Fasa, Iran , Ai, Jafar Department of Tissue Engineering and Applied Cell Sciences - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
Objective(s): Bone tissue engineering is aimed at the fabrication of bone graft to ameliorate bone defects
without using autografts or allografts.
Materials and Methods: In the present study, the coprecipitation method was used to prepare hydroxyapatite
(HA) nanoparticles containing nandrolone. To do so, 12.5, 25, and 50 mg of nandrolone were loaded into
poly(lactic acid) (PLA)/nano-HA, and the freeze casting method was used to fabricate porous scaffolds.
The morphology, mechanical strength, wettability, porosity, degradation, blood compatibility, and cellular
response of the scaffolds were evaluated using various tests. For further investigation, the developed scaffolds
were incorporated into the rat calvaria defect model, and their effects on bone healing were evaluated.
Results: The obtained results indicated that the fabricated scaffolds had the approximate porosity of 80% and
compress strength of 6.5 MPa. Moreover, the prepared scaffolds had appropriate hydrophilicity, weight loss,
and blood compatibility. Furthermore, the histopathological findings demonstrated that the defects filled
with the PLA/nano-HA scaffolds containing 25 mg nandrolone healed better compared to the other study groups.
Conclusion: Therefore, it was concluded that the scaffolds containing nandrolone could be used in bone
regeneration.
