Author/Authors :
Samadi, Mahsan Isfahan Student Research Center - Isfahan University of Medical Sciences, Isfahan, Iran , Tabibian, Farinaz Isfahan Student Research Center - Isfahan University of Medical Sciences, Isfahan, Iran , Moradzadeh, Kobra Department of Genetics and Molecular Biology - Isfahan University of Medical Sciences, Isfahan, Iran , Nassiri, Mahdi Department of Clinical Pathology - Faculty of Veterinary Medicine - University of Tehran, Tehran, Iran , Gheisari, Yousof Department of Genetics and Molecular Biology - Isfahan University of Medical Sciences, Isfahan, Iran
Abstract :
Background: Acute kidney injury is a high‑risk complication in a variety of clinical situations mostly due to ischemia–reperfusion (IR)
injuries. The novel idea of remote ischemic preconditioning (rIPC) was proposed to prevent serious ischemia sequels. To
address the controversy of previous reports, the current study was performed to assess the effect of rIPC on kidney IR injury.
Materials and Methods: Male BALB/c mice were exposed to either rIPC or sham intervention, 24 h before kidney IR. In two independent
sets of experiments, rIPC was accomplished by inducing three cycles of 5 min ischemia with 5 min reperfusion intervals through the
ligation of the left external iliac artery or infrarenal abdominal aorta. Kidney IR injury was performed by left renal pedicle occlusion
for 35 min and simultaneous right nephrectomy. After 48 h, mice were sacrificed for the assessment of kidney function and structure.
Results: According to the serum urea and creatinine, as well as histopathological measures, none of the exploited rIPC procedures
could significantly protect against kidney IR injury. Conclusion: Based on our findings and the divergent results of previous animal
and human studies, it can be concluded that the renoprotective effects of rIPC are minimal, if any, and are not robustly detectable.
