Author/Authors :
Mansouri, Vahid Proteomics Research Center - Faculty of Paramedical Sciences - Shahid Beheshti University of Medical Sciences, Tehran , Razzaghi, Mohammadreza Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Rostami-Nejad, Mohammad Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran , Rezaei-Tavirani, Majid Firoozabadi Hospital - Faculty of Medicine - Iran University of Medical Sciences, Tehran , Heidari, Mohammad Hossein Proteomics Research Center - Faculty of Paramedical Sciences - Shahid Beheshti University of Medical Sciences, Tehran , Safari, Saeed Proteomics Research Center - Department of Emergency Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Arjmand, Babak Cell Therapy and Regenerative Medicine Research Center - Endocrinology and Metabolism Molecular-Cellular Sciences Institute - Tehran University of Medical Sciences , Rezaei-Tavirani, Mostafa Proteomics Research Center - Faculty of Paramedical Sciences - Shahid Beheshti University of Medical Sciences, Tehran , Zali, Alireza Functional Neurosurgery Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Hamdieh, Mostafa Department of Psychosomatic - Taleghani Hospital - Faculty of Medicine - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Introduction: Photobiomodulation (PBM) is known as low-level laser (or light) therapy and is applied in different fields of medicine. However, it is required that its molecular and cellular mechanism be investigated. This study aims to assess the neuroprotective properties of PBM in the rat retina.
Methods: GSE22818 was downloaded from Gene Expression Omnibus (GEO) and the regulation
of the significant differentially expressed genes (DEGs) which are produced by light damage in
the rat retina by the pretreatment of PBM application was assessed via network analysis and gene
ontology enrichment.
Results: The 78 produced DEGs by light-damage in the rat retina were protected via PBM
pretreatment action. Among these determined DEGs, 53 individuals were included in the main
connected component of the constructed protein-protein interaction (PPI) network. Ccl2, Icam1,
Cxcl10, Timp1, and Fos were determined as hub nodes. Eight clusters including 26 regulated
biochemical pathways by PBM pretreatment were identified. The critical DEGs based on the
action maps were introduced.
Conclusion: The finding indicates that PBM treatment protects rat retina against light damage via the prevention of Fos, Ccl2, Icam1, Cxcl10, and Myc dysregulation.
Keywords :
Photobiomodulation therapy , Retina , Gene expression , Protein-protein interaction network , Rat
