Author/Authors :
Pashapour, Shadi Department of Tuberculosis and Lung Diseases Research Center - Tabriz University of Medical Sciences, Tabriz, Iran , Shanehbandi, Dariush Department of Immunology Research Center - University Tabriz University of Medical Sciences, Tabriz, Iran , Bornehdeli, Soghra Department of Immunology Research Center - University Tabriz University of Medical Sciences, Tabriz, Iran , Zafari, Venus Department of Tuberculosis and Lung Diseases Research Center - Tabriz University of Medical Sciences, Tabriz, Iran , Mohammad Reza Khani, Haniye Department of Immunology Research Center - University Tabriz University of Medical Sciences, Tabriz, Iran , Hashemzadeh, Shahriyar Department of Immunology Research Center - University Tabriz University of Medical Sciences, Tabriz, Iran , Asvadi Kermani, Touraj Department of Surgery - Imam Reza Hospital - University Tabriz University of Medical Sciences, Tabriz, Iran
Abstract :
Background: Aberrant expression level of Hox transcript antisense intergenic RNA (HOTAIR) has been associated with the etiopathogenesis of numerous cancers. Studies on epidemiological data have demonstrated that the risk of susceptibility to colon cancer varies among different populations due to several reasons. In this study, we aimed to assess the expression level of HOTAIR in tumoral tissues of patients with colon cancer and compare it with normal marginal tissues.
Methods: In this case-control study, we recruited a total of 50 patients with colon cancer and collected tumoral and matched marginal tumor free tissues during surgery. Afterwards, we isolated the total RNA from each sample, synthesized cDNA, and performed quantitative analysis by Real-time PCR using the SYBR Green PCR Master Mix in order to measure the transcript level of HOTAIR in samples.
Results: The expression level of HOTAIR was upregulated in tumor tissues compared with normal tumor-free marginal tissues belonging to colon cancer patients (P= 0.0023). Moreover, the expression level of HOTAIR and the clinicopathological specifications of the patients had statistically significant correlations.
Conclusions: HOTAIR may play a role in the development of colon cancer and have the potential for application as a biomarker for colon cancer prognosis
Keywords :
Colorectal cancer , HOTAIR , Transcription , Cancer biomarker
