A Novel Non-enzymatic Biosensor Based on Ti‑Metallic Glass Thin Film: The Blood Glucose Oxidation Approach

Background: Material selection is a key issue for the fabrication of non-enzymatic electrode in glucose biosensors. Metallic glass (MG) as an advanced innovative material can provides many basic structural requirements of electrodes. A novel non-enzymatic biosensor based on Ti57Cu28{Zr0.95−Hf0.05}XSi15‑X MG (Ti‑MG) thin film was introduced for glucose oxidation. Methods: The Ti‑MG thin film was deposited on the carbon substrate of screen‑printed carbon electrode (SPCE), and the Ti‑MG modified SPCE was fabricated as Ti‑MG/SPCE. The morphology and structure of the Ti‑MG thin film were characterized by field emission scanning electron microscope and X‑ray diffraction. Electrochemical evaluations were studied by electrochemical impedance spectroscopy and cyclic voltammetry. Results: The Ti‑MG was sputtered on the carbon substrate in the form of a porous spongy thin film with 285 nm thickness and nanoparticles with average diameter size of 110 nm. The Ti-MG/SPCE showed low charge transfer resistance to the electron transfer and high electrocatalytic activity toward the oxidation of glucose in PBS (pH = 7.4) solution. This biosensor exhibited good analytical performance with a linear range from 2 to 8 mM glucose and sensitivity of 0.017 μA mM−1. Conclusion: The experimental results indicate that Ti‑MG thin film has a high ability to electron transfer and glucose oxidation for the development of non-enzymatic glucose biosensors