Title of article :
Alteration in Expression of Primordial Germ Cell (PGC) Markers During Induction of Human Amniotic Mesenchymal Stem Cells (hAMSCs)
Author/Authors :
Alifi, Farzane Department of Anatomical Sciences - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Asgari, Hamid Reza Department of Anatomical Sciences - School of Medicine - Iran University of Medical Sciences, Tehran, Iran
Abstract :
Background: Currently, scientists are looking for a solution to the problem of the
couples who have a lack of germ cells by through cell therapy. It is found that human
amniotic membrane mesenchymal stem cells (hAMSCs) could be a good candidate
for solving this problem. In the present study, an attempt was made to show that
hUMSCs can express the PGC markers in the presence of retinoic acid (RA).
Methods: Placenta was obtained from healthy mothers and amniotic stem cells were
isolated by enzymatic method from amniotic membrane. The cells were treated by
retinoic acid for 14 days. Mesenchymal properties of hAMSCs were assessed by
flow-cytometry and expression of PGC markers was established by Q-PCR.
Results: Mesenchymal stem cell properties were confirmed by antibodies against
mesenchymal stem cell markers (CD73, CD90, and CD105). After that, the expression
of the C-kit, Oct4, SSEA4, VASA genes were determined as primordial germ
cell markers using quantitative PCR. It was found that the use of retinoic acid led to
the highest expression of C-kit, SSEA4, VASA genes and lower expression of Oct4.
Conclusion: Our study indicates that retinoic acid can be used as a suitable factor
for induction of hAMSCs into primordial germ cells (PGCs) and hAMSCs have
enough potential to do that.
Keywords :
Amnion , Cell differentiation , Germ cells , Mesenchymal stem cells , Retinoic acid
Journal title :
Journal of Reproduction and Infertility (JRI)